First report of Banana bunchy top virus on heliconia (Heliconia spp.) in Bali, Indonesia

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Published: Mar 31, 2022
DOI: https://doi.org/10.23960/jhptt.12277-82
Keywords:
Banana Bunchy Top Diseases (BBTD) banana production molecular detection mRep

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I Made Arimbawa
Departement of Agricultural Biotechnology, Faculty of Agriculture, Universitas Udayana
Gusti Ngurah Alit Susanta Wirya
Department of Agroecotechnology, Faculty of Agriculture, Universitas Udayana
I Putu Sudiarta
Department of Agroecotechnology, Faculty of Agriculture, Universitas Udayana

Abstract

Banana bunchy top virus (BBTV) mostly infects Musa spp. which causes banana bunchy top disease (BBTD), the most devastating viral disease in banana cultivation all over the world. During field survey in Sekar Bumi Tropical Farm located in Kerta Village, 15 heliconia plant samples showed green streak on lamina leaves that similar to common BBTD symptoms were collected. Further research was then conducted to identify molecular characteristic of BBTD in heliconia showing green streak. Molecular identification was conducted using polymerase chain reaction (PCR). Specific primers to amplified DNA target were used in this study: mRep/F (5’-GCGTGAAACGCACAAAAGGCC-3’) and mRep/R (5’-GCATACGTTGTCAAACCTTCTCCTC-3’). The expected 240 bp fragment target of partial master replication (mRep) gene was successfully amplified from ten out of 15 samples. Sequence analysis confirmed that the symptomatic heliconia samples were infected with BBTV and falls into the same clade with BBTV from the Asian Group.

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(1)
Arimbawa, I. M.; Wirya, G. N. A. S.; Sudiarta, I. P. First Report of Banana Bunchy Top Virus on Heliconia (Heliconia spp.) in Bali, Indonesia. J Trop Plant Pests Dis 2022, 22, 77-82.
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Vol. 22 No. 1 (2022): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PESTS AND DISEASES
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

Amin I, Qazi J, Mansoor S, Ilyas M, & Briddon RW. 2008. Molecular characterization of Banana bunchy top virus (BBTV) from Pakistan. Virus Genes. 36(1): 191–198. https://doi.org/10.1007/s11262-007-0168-y

Beetham PR, Hafner GJ, Harding RM, & Dale JL. 1997. Two mRNAs are transcribed from Banana bunchy top virus DNA-1. J. Gen. Virol. 78(1): 229–236. https://doi.org/10.1099/0022-1317-78-1-229

Blackman RL & Eastop VE. 1984. Aphids on the Worlds Crops: An Identification and Information Guide. Second Edition. John Wiley & Sons, Chichester.

Burns TM, Harding RM, & Dale JL. 1995. The genome organization of Banana bunchy top virus: analysis of six ssDNA components. J. Gen. Virol. 76(6): 1471–1482. https://doi.org/10.1099/0022-1317-76-6-1471

Chiaki Y, Nasir N, Herwina H, Jumjunidang, Sonoda A, Fukumoto T, Nakamura M, & Iwai M. 2015. Genetic structure and diversity of the Banana bunchy top virus population on Sumatra Island, Indonesia. Eur. J. Plant Pathol. 143(1): 113–122. https://doi.org/10.1007/s10658-015-0669-9

Furuya N, Somowiyarjo S, & Natsuati KT. 2004. Virus detection from local banana cultivars and the ?rst molecular characterization of Banana bunchy top virus in Indonesia. Jour. Agri. Sci. Tokyo Univ. of Agric. 49(3): 75–81.

Geering ADW & Thomas JE. 1997. Search for alternative hosts of Banana bunchy top virus in Australia. Australas. Plant Pathol. 26(4): 250–254. https://doi.org/10.1071/AP97040

Hafner GJ, Harding RM, & Dale JL. 1997. A DNA primer associated with Banana bunchy top virus. J. Gen. Virol. 78(2): 479–486. https://doi.org/10.1099/0022-1317-78-2-479

Hamim I, Green JC, Borth WB, Melzer MJ, Wang YN, & Hu JS. 2017. First report of Banana bunchy top virus in Heliconia spp. on Hawaii. Plant Dis. 101(12): 1–2. https://doi.org/10.1094/PDIS-02-17-0205-PDN

Horser CL, Harding RM, & Dale JL. 2001. Banana bunchy top nanovirus DNA-1 encodes the ‘master’ replication initiation protein. J. Gen. Virol. 82(2): 459–464. https://doi.org/10.1099/0022-1317-82-2-459

Hu JS, Wang M, Sether D, Xie W, & Leonhardt KW. 1996. Use of polymerase chain reaction (PCR) to study transmission of Banana bunchy top virus by the banana aphid (Pentalonia nigronervosa). Ann. Appl. Biol. 128(1): 55–66. https://doi.org/10.1111/j.1744-7348.1996.tb07089.x

Kakathi N & Nath PD. 2018. Genetic diversity of Banana bunchy top virus (BBTV) prevalent in Assam causing banana bunchy top disease. Int. J. Curr. Microbiol. App. Sci. 7(11): 1547–1560. https://doi.org/10.20546/ijcmas.2018.711.178

Kress J. 1990. The diversity and distribution of Heliconia (Heliconiaceae) in Brazil. Acta Bot. Bras. 4(1): 159–167. https://doi.org/10.1590/S0102-33061990000100011

Kumar S, Stecher G, & Tamura K. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7): 1870–1874. https://doi.org/10.1093/molbev/msw054

Kumar P, Arun V, & Lokeswari TS. 2017. Cloning of BBTV (Banana bunchy top virus) components and screening of BBTV using functionalized gold nanoparticles. 3 Biotech. 7(3): 225. https://doi.org/10.1007/s13205-017-0849-8

Leiwakabessy M, Nurulita S, & Hidayat SH. 2017. Disease incidence and molecular analysis of Banana bunchy top virus in Bogor, West Java. In: Efendi D & Maharijaya A (Eds.). Proceeding International Seminar on Tropical Horticulture 2016. pp. 37–45. Pusat Kajian Hortikultura Tropika–LPPM IPB, Bogor.

Mansoor S, Qazi J, Amin I, Khatri A, Khan IA, Raza S, Zafar Y, & Briddon RW. 2005. A PCR-based method, with internal control, for the detection of Banana bunchy top virus in banana. Mol. Biotechnol. 30(2): 127–129. https://doi.org/10.1385/MB:30:2:167

Pinili MS, Nyana DN, Suastika G, & Natsuaki KT. 2011. Molecular analysis of Banana bunchy top virus first isolated in Bali, Indonesia. Journal of Agricultural Science, Tokyo University of Agriculture. 56(2): 125–134.

Rahim YF, Damayanti TA, & Ghulamahdi M. 2015. Deteksi virus yang menginfeksi kedelai di Jawa [Detection of viruses infecting soybean in Java]. Jurnal Fitopatologi Indonesia. 11(2): 59–67. https://doi.org/10.14692/jfi.11.2.68

Stainton D, Martin DP, Muhire BM, Lolohea S, Halafihi M, Lepoint P, Blomme G, Crew KS, Sharman M, Kraberger S, Dayaram A, Walters M, Collings DA, Mabvakure B, Lemey P, Harkins GW, Thomas JE, & Varsani A. 2015. The global distribution of Banana bunchy top virus reveals little evidence for frequent recent, human-mediated long distance dispersal events. Virus Evol. 1(1): vev009. https://doi.org/10.1093/ve/vev009

Thomas JE. 2008. Banana bunchy top virus. In: Mahy BWJ & Van Regenmortel MHV (Eds.). Encyclopedia of Virology (Third Edition). pp. 272–279. Elsevier, Oxford. https://doi.org/10.1016/B978-012374410-4.00636-1

Watanabe S, Greenwell AM, & Bressan A. 2013. Localization, concentration, and transmission efficiency of Banana bunchy top virus in four asexual lineage of Pentalonia aphids. Viruses. 5(2): 758–775. https://doi.org/10.3390/v5020758

Wickramaarachchi WART, Shankarappa KS, Rangaswamy KT, Maruthi MN, Rajapakse RGAS, & Ghosh S. 2016. Molecular characterization of Banana bunchy top virus isolated from Sri Lanka and its genetic relationship with other isolates. VirusDis. 27(2): 154–160. https://doi.org/10.1007/s13337-016-0311-2

Yasmin T, Khalid S, Soomro MH, Malik SA, Shah H, & Ahmad I. 2001. Specificity of host-pathogen interaction of banana bunchy top disease. J. Biol. Sci. 1(4): 212–213. https://doi.org/10.3923/jbs.2001.212.213